Methodology guides for reviewable video analysis.
Practical explanations of calibration, locomotor metrics, spatial zones, immobility and tracking quality—written for researchers evaluating their own protocol.
Open Field Test Metrics: What to Measure
Understand trajectory, distance, speed, center/periphery occupancy, spatial occupancy and immobility.
Read guide RECORDING GUIDEHow to Record Mouse Open Field Videos for Automated Tracking
Practical guidance for camera position, arena visibility, lighting, contrast and stable recording conditions.
Read guide Y-MAZE METHODOLOGYHow to Calculate Spontaneous Alternation in a Y-Maze
The exact RodentMetrics formula, a worked example, edge cases and what the percentage does and does not show.
Read guide EPM METHODOLOGYHow EPM Arm Entries, Time and Head Dip Are Calculated
Zone occupancy, arm-entry confirmation, dwell time, and why Head Dip is a separate diagnostic measure, not an official metric.
Read guide NOR METHODOLOGY · VALIDATION-ONLY MEASUREHow NOR Discrimination Index Is Calculated
The exact formula behind the experimental, pose-derived Novel Object Recognition discrimination measure, a worked example, and what it does not establish.
Read guide MWM METHODOLOGY · VALIDATION-ONLY MEASURESHow MWM Escape Latency Is Calculated
The exact platform-reach rule and escape-latency formula for the supported single-trial workflow, including current validation and scope limitations.
Read guide ANALYSIS TRANSPARENCYAnalysis Reproducibility and Methodology
What reproducibility evidence RodentMetrics can defensibly support, what can change a result, and why repeatability is a different question from scientific validation.
Read guide OPEN FIELD TEST GUIDEHow to analyze an Open Field Test from video
A defensible analysis starts before a metric is calculated. Define the arena and protocol, calibrate the recording, review tracking quality and only then interpret locomotor and spatial outputs.
Read guide SPATIAL ANALYSISCenter vs periphery: define the zone before interpreting it
Center-periphery results are operational measurements. Their meaning depends on the arena, center-zone geometry, session design and the biological question.
Read guide LOCOMOTOR METRICSDistance and speed in rodent video tracking
Distance and speed look simple, but calibration, frame timing, detection gaps and noise handling materially affect the result.
Read guide ACTIVITY CLASSIFICATIONImmobility analysis requires explicit thresholds
Video-based immobility is an operational state derived from estimated speed—not a direct observation of a biological mechanism.
Read guide QUALITY CONTROLHow to validate rodent video tracking quality
Summary metrics are only as credible as the detections beneath them. Tracking review should be a documented part of every analysis.
Read guideValidate the workflow for your protocol
Tracking quality depends on camera position, arena visibility, contrast, lighting, occlusion and configured thresholds. RodentMetrics does not infer anxiety, diagnose conditions or replace manual quality review. Report protocol choices, inspect gaps and retain independent source-data backups.