Prepare and calibrate
Upload an overhead video, mark the arena corners and enter physical dimensions when distance and speed in centimeters are required.
OPEN FIELD TEST ANALYSIS
From overhead video to reviewable open field metrics.
Calibrate the arena, inspect single-animal tracking and quantify locomotion, spatial occupancy, center-periphery behavior and immobility in one guided workflow.
Designed for single-animal overhead recordings in a clearly visible arena.1234
Reviewable outputsTrajectoryOccupancyMetricsIllustrative interface preview. Values are not experimental claims.
A transparent Open Field Test workflow
Configuration is explicit, outputs remain connected to the source analysis and researchers can inspect the tracking before interpretation.
Track movement
Background-subtraction-based tracking estimates the animal centroid frame by frame within the calibrated arena.
Configure spatial measures
Choose grid dimensions and operational center-zone boundaries appropriate to the study protocol.
Define activity states
Configure speed smoothing, an immobility threshold and minimum bout duration; missing detections interrupt episodes.
Review quality
Inspect detections and gaps against the video before relying on summary statistics.
Export a research record
Download frame-level CSV data and a PDF containing methods, settings, metrics and figures.
Mouse Open Field analysis resources
Explore the dedicated analysis software page for the complete browser-based Mouse Open Field workflow.
SCIENTIFIC LIMITATIONS
Validate the workflow for your protocol
Tracking quality depends on camera position, arena visibility, contrast, lighting, occlusion and configured thresholds. RodentMetrics does not infer anxiety, diagnose conditions or replace manual quality review. Report protocol choices, inspect gaps and retain independent source-data backups.